Development of a cytochrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices.
Identifieur interne : 000121 ( France/Analysis ); précédent : 000120; suivant : 000122Development of a cytochrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices.
Auteurs : Montserrat Cortina-Puig [France] ; Xavier Mu Oz-Berbel ; Regis Rouillon ; Carole Calas-Blanchard ; Jean-Louis MartySource :
- Bioelectrochemistry (Amsterdam, Netherlands) [ 1878-562X ] ; 2009.
English descriptors
- KwdEn :
- Animals, Antioxidants (analysis), Antioxidants (chemistry), Antioxidants (metabolism), Beverages, Biosensing Techniques (methods), Cattle, Citrus sinensis (chemistry), Cytochromes c (chemistry), Cytochromes c (metabolism), Electrochemistry, Electrodes, Enzymes, Immobilized (chemistry), Enzymes, Immobilized (metabolism), Gold (chemistry), Gold (metabolism), Horses, Printing, Superoxides (chemistry), Superoxides (metabolism), Xanthine Oxidase (chemistry), Xanthine Oxidase (metabolism).
- MESH :
- chemical , analysis : Antioxidants.
- chemical , chemistry : Antioxidants, Cytochromes c, Enzymes, Immobilized, Gold, Superoxides, Xanthine Oxidase.
- chemical , metabolism : Antioxidants, Cytochromes c, Enzymes, Immobilized, Gold, Superoxides, Xanthine Oxidase.
- chemistry : Citrus sinensis.
- methods : Biosensing Techniques.
- Animals, Beverages, Cattle, Electrochemistry, Electrodes, Horses, Printing.
Abstract
This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices.
DOI: 10.1016/j.bioelechem.2009.04.004
PubMed: 19447685
Affiliations:
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pubmed:19447685Le document en format XML
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Perpignan</orgName></affiliation></author><author><name sortKey="Mu Oz Berbel, Xavier" sort="Mu Oz Berbel, Xavier" uniqKey="Mu Oz Berbel X" first="Xavier" last="Mu Oz-Berbel">Xavier Mu Oz-Berbel</name></author><author><name sortKey="Rouillon, Regis" sort="Rouillon, Regis" uniqKey="Rouillon R" first="Regis" last="Rouillon">Regis Rouillon</name></author><author><name sortKey="Calas Blanchard, Carole" sort="Calas Blanchard, Carole" uniqKey="Calas Blanchard C" first="Carole" last="Calas-Blanchard">Carole Calas-Blanchard</name></author><author><name sortKey="Marty, Jean Louis" sort="Marty, Jean Louis" uniqKey="Marty J" first="Jean-Louis" last="Marty">Jean-Louis Marty</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19447685</idno><idno type="pmid">19447685</idno><idno type="doi">10.1016/j.bioelechem.2009.04.004</idno><idno type="wicri:Area/PubMed/Corpus">000929</idno><idno 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France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IMAGES EA 4218, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName><orgName type="university">Université de Perpignan</orgName></affiliation></author><author><name sortKey="Mu Oz Berbel, Xavier" sort="Mu Oz Berbel, Xavier" uniqKey="Mu Oz Berbel X" first="Xavier" last="Mu Oz-Berbel">Xavier Mu Oz-Berbel</name></author><author><name sortKey="Rouillon, Regis" sort="Rouillon, Regis" uniqKey="Rouillon R" first="Regis" last="Rouillon">Regis Rouillon</name></author><author><name sortKey="Calas Blanchard, Carole" sort="Calas Blanchard, Carole" uniqKey="Calas Blanchard C" first="Carole" last="Calas-Blanchard">Carole Calas-Blanchard</name></author><author><name sortKey="Marty, Jean Louis" sort="Marty, Jean Louis" uniqKey="Marty J" first="Jean-Louis" last="Marty">Jean-Louis Marty</name></author></analytic><series><title level="j">Bioelectrochemistry (Amsterdam, Netherlands)</title><idno type="eISSN">1878-562X</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antioxidants (analysis)</term><term>Antioxidants (chemistry)</term><term>Antioxidants (metabolism)</term><term>Beverages</term><term>Biosensing Techniques (methods)</term><term>Cattle</term><term>Citrus sinensis (chemistry)</term><term>Cytochromes c (chemistry)</term><term>Cytochromes c (metabolism)</term><term>Electrochemistry</term><term>Electrodes</term><term>Enzymes, Immobilized (chemistry)</term><term>Enzymes, Immobilized (metabolism)</term><term>Gold (chemistry)</term><term>Gold (metabolism)</term><term>Horses</term><term>Printing</term><term>Superoxides (chemistry)</term><term>Superoxides (metabolism)</term><term>Xanthine Oxidase (chemistry)</term><term>Xanthine Oxidase (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antioxidants</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antioxidants</term><term>Cytochromes c</term><term>Enzymes, Immobilized</term><term>Gold</term><term>Superoxides</term><term>Xanthine Oxidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antioxidants</term><term>Cytochromes c</term><term>Enzymes, Immobilized</term><term>Gold</term><term>Superoxides</term><term>Xanthine Oxidase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Beverages</term><term>Cattle</term><term>Electrochemistry</term><term>Electrodes</term><term>Horses</term><term>Printing</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Perpignan</li></settlement><orgName><li>Université de Perpignan</li></orgName></list><tree><noCountry><name sortKey="Calas Blanchard, Carole" sort="Calas Blanchard, Carole" uniqKey="Calas Blanchard C" first="Carole" last="Calas-Blanchard">Carole Calas-Blanchard</name><name sortKey="Marty, Jean Louis" sort="Marty, Jean Louis" uniqKey="Marty J" first="Jean-Louis" last="Marty">Jean-Louis Marty</name><name sortKey="Mu Oz Berbel, Xavier" sort="Mu Oz Berbel, Xavier" uniqKey="Mu Oz Berbel X" first="Xavier" last="Mu Oz-Berbel">Xavier Mu Oz-Berbel</name><name sortKey="Rouillon, Regis" sort="Rouillon, Regis" uniqKey="Rouillon R" first="Regis" last="Rouillon">Regis Rouillon</name></noCountry><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Cortina Puig, Montserrat" sort="Cortina Puig, Montserrat" uniqKey="Cortina Puig M" first="Montserrat" last="Cortina-Puig">Montserrat Cortina-Puig</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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